Device

Part:BBa_K1260008:Design

Designed by: Zhengfeng Liu   Group: iGEM14_Shenzhen_SFLS   (2014-05-24)


pbad- ScFv(nonsp) (whole device)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A L-arabinose promoter, a strong RBS B0030 and a double termintor has been added to the CDS of ScFv without signal peptide.

Source

It comes from BBa_K1260007 and BBa_K206001.

References